Preprints
Endogenous EWSR1-FLI1 degron alleles enable control of fusion oncoprotein expression in tumor cell lines and xenografts. McGinnis JH, Enriquez AB, Vandiver F, Bai X, Kim J, Kilgore J, Saha P, O’Hara R, Xie Y, Banaszynski LA, Williams N, McFadden DG. (2024). bioRxiv
G1 length dictates heterochromatin landscape. Trouth A, Ravichandran K, Gafken P, Martire S, Namciu SJ, Banaszynski LA, Sarthy JF, Ramachandran S. (2024). bioRxiv
Loss of heterochromatin at endogenous retroviruses creates competition for transcription factor binding. O’Hara R and Banaszynski LA. (2022). bioRxiv
UT Southwestern
Acute MeCP2 loss in adult mice reveals transcriptional and chromatin changes that precede neurological dysfunction and inform pathogenesis. Bajikar SS, Zhou J, O’Hara R, Tirumala HP, Durham MA, Trostle AJ, Dias M, Shao Y, Chen H, Wang W, Yalamanchili HK, Wan Y-W, Banaszynski LA, Liu Z, Zoghbi HY. (2024). Neuron
TASOR expression in naive embryonic stem cells safeguards their developmental potential. Pinzon-Arteaga CA*, O’Hara R*, Mazzagati A, Ballard E, Hu Y, Pan A, Schmitz DA, Wei Y, Sakurai M, Ly P, Banaszynski LA#, Wu J#. (2024). Cell Reports 43, 114887. *equal contribution #co-corresponding
PASK links cellular energy metabolism with a mitotic self-renewal network to establish differentiation competence. Xiao M, Wu CH, Meek G, Kelly B, Young LEA, Martire S, Buendia Castillo D, Saha P, Dube AL, Gentry MS, Banaszynski LA, Sun RC, Kikani CK. (2023). Elife, e81717.
H3.3 contributes to chromatin accessibility and transcription factor binding at promoter-proximal regulatory elements. Tafessu A*, O’Hara R*, Martire S*, Dube AL, Saha P, Gant VU, Banaszynski LA. (2023). Genome Biology 24, 25. *equal contribution
Chromatin accessibility analysis from fresh and cryopreserved human ovarian follicles. Shannon J, Sundaresan A, Bukulmez O, Jiao Z, Doody K, Capelouto S, Carr B, Banaszynski LA. (2022). Mol. Hum. Reprod. doi: 10.1093/molehr/gaac020
Oncohistone Mutations Occur at Functional Sites of Regulatory ADP-ribosylation. Huang D, Camacho CV, Martire S, Nagari A, Setlem R, Gong X, Edwards AD, Chiu SP, Banaszynski LA, Kraus WL. (2022). Cancer Research 82, 2361.
ATRX promotes heterochromatin formation to protect cells from G quadruplex DNA-mediated stress. Teng Y-C, Sundaresan A, O’Hara R, Gant VU, Li M, Martire S, Warshaw JN, Basu A, Banaszynski LA. (2021). Nature Communications 12, 3887.
Establishment and function of chromatin modification at enhancers. Tafessu A, Banaszynski LA. (2020). Open Biology 200255. (Review)
Differential contribution of p300 and CBP to regulatory elements in mESCs. Martire S, Nguyen J, Sundaresan A, Banaszynski LA. (2020). BMC Mol. Cell. Biol. 21, 55.
The role of histone variants in fine-tuning chromatin organization and function. Martire S, Banaszynski LA. (2020). Nat. Rev. Mol. Cell Biol. 21, 522-541. (Review)
Phosphorylation of histone H3.3 at serine 31 promotes p300 activity and enhancer acetylation. Martire S, Gogate AA, Whitmill A, Tafessu A, Nguyen J, Teng Y-C, Tastemel M, Banaszynski LA. (2019). Nature Genetics. 51, 941-946.
Transcription pausing regulates mouse embryonic stem cell differentiation. Tastemel M, Gogate AA, Malladi VS, Nguyen K, Mitchell C, Banaszynski LA, Bai X. (2017). Stem Cell Res. 25, 250-255.
Checks and balances: Rpd3 issues executive orders in developmental enhancer regulation. Martire S, Banaszynski L. (2017). Dev. Cell 40, 325-326. (Preview)
Histone H3.3 is required for endogenous retroviral element silencing in embryonic stem cells. Elsässer SJ, Noh K-M, Diaz N, Allis CD, Banaszynski LA. (2015). Nature 522, 240-244. *Correction here.
Postdoctoral Work with David Allis at The Rockefeller University
Histone variant H3.3-mediated chromatin remodeling is essential for paternal genome activation in mouse preimplantation embryos. Kong Q, Banaszynski LA, Geng F, Zhang X, Zhang J, Zhang H, O'Neill CL, Yan P, Liu Z, Shido K, Palermo GD, Allis CD, Rafii S, Rosenwaks Z, Wen D. (2018). J. Biol. Chem. 293, 3829-3838.
Targeting the endogenous h3f3b (H3.3B) locus for a reporter of gene expression in mice. Wen D, Noh K-M, Golberg AD, Allis CD, Rosenwaks Z, Rafii S, Banaszynski LA. (2014). Genesis 52, 959-966.
H3.3 replacement facilitates epigenetic reprogramming of donor nuclei in somatic cell nuclear transfer (SCNT) embryos. Wen D, Banaszynski LA, Rosenwaks Z, Allis CD, Rafii S. (2014). Nucleus 5, 369-375.
Histone variant H3.3 is an essential maternal factor for oocyte reprogramming. Wen D, Banaszynski LA, Liu Y, Xiang J, Elemento O, Rosenwaks Z, Allis CD, Rafii S. (2014). Proc. Natl. Acad. Sci. 111, 7325-7330.
Hira-dependent histone H3.3 deposition facilitates PRC2 recruitment at developmental loci in ES cells. Banaszynski LA, Wen D, Dewell S, Whitcomb SJ, Lin M, Diaz N, Elsässer SJ, Chapgier A, Goldberg AD, Canaani E, Rafii S, Zheng D, Allis CD. (2013). Cell 155, 107-120.
Inhibition of PRC2 activity by gain-of-function H3 mutants found in pediatric glioblastomas. Lewis PW, Muller MM, Koletsky MS, Cordero F, Lin S, Banaszynski LA, Garcia BA, Muir TW, Becher OJ, Allis CD. (2013). Science 340, 857-861.
Distinct factors control histone variant H3.3 localization at specific genomic regions. Goldberg, AD, Banaszynski LA, Noh K-M, Lewis PW, …, Allis CD. (2010). Cell 140, 678-691.
Histone variants in metazoan development. Banaszynski LA, Allis CD, and Lewis PW. (2010). Dev. Cell 19, 662-674. (Review)
Analysis of histones and chromatin in Xenopus laevis egg and oocyte extracts. Banaszynski LA, Allis CD, Shechter D. (2010). Methods 51, 3-10
Graduate Work with Tom Wandless at Stanford UNIVERSITY
A general method for conditional regulation of protein stability in living animals. Sellmyer, M.A., Thorne, S.H., Banaszynski, L.A., Contag, C.H., and Wandless, T.J. (2009). CSH Protocols doi: 10.1101/pdb.prot5173.
Regulating protein stability in mammalian cells using small molecules. Hagan, E.L., Banaszynski, L.A., Chen, L.-C., Maynard-Smith, L.A., and Wandless, T.J. (2009). CSH Protocols doi: 10.1101/pdb.prot5172.
Chemical control of protein stability and function in living animals. Banaszynski, L.A., Sellmyer, M.A., Contag, C.H., Wandless, T.J., Thorne, S.H. (2008). Nat. Med. 14, 1123-1127.
Recent progress with FKBP-derived destabilizing domains. Chu, B.W., Banaszynski, L.A., Chen, L.-C., Wandless, T.J. (2008). Bioorg. Med. Chem. Lett. 18, 5941-5944.
Synthesis and analysis of stabilizing ligands for FKBP-derived destabilizing domains. Grimley, J.S., Chen, D.A., Banaszynski, L.A., Wandless, T.J. (2008). Bioorg. Med. Chem. Lett. 18, 759-761.
A directed approach for engineering conditional protein stability using biologically silent small molecules. Maynard-Smith, L.A., Chen, L.-C., Banaszynski, L.A., Ooi, A.G.L., Wandless, T.J. (2007). J. Biol. Chem. 282, 24866-24872.
SIK1 is a class II HDAC kinase that promotes survival of skeletal myocytes. Berdeaux, R., Goebel, N., Banaszynski, L., Takemori, H., Wandless, T., Shelton, G.D., Montminy, M. (2007). Nat. Med. 13, 597-603.
A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Banaszynski, L.A., Chen, L.-C., Maynard-Smith, L.A., Ooi, A.G.L., Wandless, T.J. (2006). Cell 126, 995-1004.
Conditional control of protein function. Banaszynski, L.A., Wandless, T.J. (2006). Chem. Biol. 13, 11-21. (Review)
Characterization of the FKBP•rapamycin•FRB ternary complex. Banaszynski, L.A., Liu, C.W., Wandless, T.J. (2005). J. Am. Chem. Soc. 127, 4715-4721
Undergraduate Work at The University of Notre Dame
Using hydrogen bonding to control carbamate C-N rotamer equilibria. Moraczewski, A.L., Banaszynski, L.A., From, A.M., White, C.E., Smith, B.D. (1998). J. Org. Chem. 63, 7258-7262.